Fig. 6

MZptk7^hsc9 embryos establish normal dorsoventral pattern. (A) Chordin (chd) and bozozok (boz) expression is slightly increased in MZptk7^hsc9 embryos immediately following MBT, as assayed by qRT-PCR of embryos at 4 hpf. ^**P<0.01. (B) Boz expression is increased in MZptk7^hsc9 embryos prior to gastrulation as assayed by WISH (dorsal view), and by qRT-PCR at 50% epiboly (^**P=0.0078). (C) Whole-mount in situ hybridisation (WISH) for dorsal organiser genes chordin (chd) and goosecoid (gsc), and ventral genes axin2 and vox at shield stage. Embryos are viewed from the animal pole with dorsal to the right (for chd, axin2 and vox). gsc expression is shown as a dorsal view. (D) qRT-PCR assays of chd (P=0.4675), gsc (P=0.9515), axin2 (P=0.07) and vox (P=0.3974) expression in wild-type (WT) versus MZptk7^hsc9 embryos at shield stage. No significant differences in gene expression were observed. Error bars represent the s.e. for the expression level fold change.