FIGURE

Fig. 6

ID

ZDB-FIG-130426-27

Publication

Dooley et al., 2013 - On the embryonic origin of adult melanophores: the role of ErbB and Kit signalling in establishing melanophore stem cells in zebrafish

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Fig. 6

NC migration along the ventromedial path is blocked by inhibition of ErbB receptors. (A-B′′) Tg(sox10:gfp; nbt:DsRed) zebrafish embryos at 24 hpf. (A,B) Red and green channel (merge); (A′,B′) green channel; (A′′,B′′) red channel. Medial NC cells (green) covering the primary motor axons (white arrowheads, A-A′′) are absent after treatment with the ErbB inhibitor PD168393 (B-B′′). (C,D) Confocal images of 36 hpf Tg(mitfa:gfp; nbt:DsRed) embryos. (C) Wild-type embryo. (D) Embryo treated with ErbB inhibitor at 16 hpf. (E) Tg(mitfa:gfp) embryos were injected with a double MO combination against mitfa and erbb3b. Larvae were stained at 8 dpf using anti-GFP (green) and anti-HU (red) antibody (white arrowheads). The association of DRGs (red) and GFP-positive cells was quantified (Table 1). In double mitfa and erbb3b knockdowns, of 82 metamers lacking HU positive cells only five (6%) develop a string of GFP-positive cells.

Expression Data

Genes:	DsRed GFP
Fish:	ba2Tg; zf148Tg
Condition:	chemical treatment: PD 168393
Anatomical Terms:	neural crest cell neuron primary motor neuron
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ba2Tg; zf148Tg w47Tg + MO1-erbb3b + MO2-mitfa
Condition:	chemical treatment: PD 168393
Knockdown Reagents:	MO1-erbb3b MO2-mitfa
Observed In:	dorsal root ganglion melanoblast trunk neural crest cell ventral trunk neural crest cell migration
Stage Range:	Prim-5 to Days 7-13

Phenotype Detail

Acknowledgments

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