Fig. 3

KV lumen inflation requires cftr. (A-F) KV lumen (arrow), imaged at 10 ss by DIC microscopy in WT (A,C,E) and mutant siblings (B,D,F respectively). (B,D) The KV lumen is undetectable by DIC in cftr^pd1049 and cftr^pd1050 mutants at 10 ss. (F) DIC imaging of the cftr^pd1048 tailbud identified a small KV lumen. (G-J) Live confocal images showing ventral cross-section and orthogonal views of WT and mutant KV expressing Tg(sox17:GFP) and Arl13b-mCherry RNA at 10 ss in (G,H) WT and cftr^pd1049 mutant siblings and (I,J) WT and cftr^pd1048 siblings. (K,L) Confocal images of WT and mutant KV stained for acetylated tubulin. (M) Quantification of the number of KV cilia. WT KV contained 57.0±4.1 cilia (n=5) and mutant KV contained 54.5±4.6 cilia (n=8) (P=0.69). (N) Quantification of cilia length in WT and mutant KV. WT cilia length was 5.97±0.13 μm (n=30) and cftr^pd1049 cilia length was 5.82±0.13 μm (n=49) (P=0.42). Error bars represent s.e.m. Scale bars: 50 μm.