FIGURE

Fig. 6

ID

ZDB-FIG-130326-49

Publication

Djiotsa et al., 2012 - Pax4 is not essential for beta-cell differentiation in zebrafish embryos but modulates alpha-cell generation by repressing arx gene expression

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Fig. 6

Up-regulation and nuclear localization of pax4 and arx altered transcript. (A–D) Confocal images of the pancreas area of 30 hpf embryos co-stained for pax4 mRNA (green) and Topro3 (blue) to visualize all cell nuclei, in control morphants (A-C) and pax4 morphants (B-D). Scale bar = 20 μm. (E-H) Confocal images of the pancreas area of 30 hpf embryos analyzed by fluorescent WISH with arx probe (red) and stained for Topro3 (blue). arx expression in somites and the floor plate of control morphants (E, G) and of arx morphants (F, H). Scale bar = 20 μm. (I-L) Ventral view of WISH 30 hpf embryos showing upregulation of arx transcripts in somites and floor plate in Moarx (J) and MoTarx (L) morphants. Original magnification 200x. (M – P) Ventral views of the pancreas area from embryos analyzed by WISH showing expression of pax4 in control morphants (M), pax4 morphants (N), arx morphants (O) and double pax4/arx morphants (P). Original magnification 400x. Anterior part of all the embryos is in the left. Asterisks indicate arx positive cells. fp: floor plate, s: somite, cont: control morphant.

Expression Data

Genes:	arxa pax4
Fish:	WT WT + MO1-pax4 WT + MO3-arxa WT + MO4-arxa WT + MO1-pax4 + MO3-arxa
Knockdown Reagents:	MO1-pax4 MO3-arxa MO4-arxa
Anatomical Terms:	floor plate pancreatic bud somite
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.