FIGURE

Fig. 2

ID

ZDB-FIG-130326-42

Publication

Djiotsa et al., 2012 - Pax4 is not essential for beta-cell differentiation in zebrafish embryos but modulates alpha-cell generation by repressing arx gene expression

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Fig. 2

Identification of pax4 and arx expressing cells by double fluorescent in situ hybridization. All images are confocal optical sections of the dorsal pancreatic bud with anterior part to the left. The probes and the developmental stages are respectively indicated at the top and the bottom in each image. All views are ventral, except images P, Q, R and S which are lateral views. arx transcripts are detected in almost all glucagon+ (A, B), in some ghrelin+ (C, D) cells and in many isl1+ cells (H); absence of co-staining between arx and insulin (E, F) and somatostatin (G). pax4 transcripts are not detected in insulin+ (I - K) and most glucagon+ cells (L). pax4 is expressed in many somatostatin (M) and a few ghrelin (N) expressing cells. Partial co-staining of pax4 probe with a cocktail of insulin, glucagon, somatostatin and ghrelin probes (horm)(O), with isl1 (P) and sox4b (Q) probes. Absence of co-staining between pax4 and nkx6.1 (R). S: Schematic representation of the multi-layer organization of the pancreatic dorsal bud at 24 hpf including pax4 expression. Scale bar = 20 μm. astérisks (*) indicate double positive cells.

Expression Data

Genes:	arxa gcga ghrl ins isl1a nkx6.1 pax4 sox4b sst2
Fish:	WT
Anatomical Terms:	D cell ghrelin secreting cell glucagon secreting cell insulin secreting cell pancreatic A cell pancreatic B cell pancreatic bud pancreatic epsilon cell posterior pancreatic bud somatostatin secreting cell
Stage Range:	14-19 somites to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.