FIGURE

Fig. 3

ID

ZDB-FIG-130304-19

Publication

Bohnsack et al., 2013 - Thyroid hormone and retinoic acid interact to regulate zebrafish craniofacial neural crest development

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Fig. 3

TH regulated craniofacial muscle development including EOM organization. 96 hpf control embryos demonstrated 6 distinct EOM; medial rectus (MR), lateral rectus (LR), superior rectus (SR), superior oblique (SO), inferior rectus (IR), and inferior oblique (IO) in vivo in Tg(α-actin::EGFP) (A, D) and by in situ hybridization for myzh2 (G, I). In situ hybridization for myoD demonstrated early muscle differentiation in EOM at 60 hpf (K, M). Thraa MO knockdown inhibited jaw and PA muscle development (B, H). Thraa MO knockdown did not disrupt muscle differentiation, but caused indistinct superior (sup) and inferior (inf) EOM complexes (E, H, J). Dio3 MO knockdown inhibited posterior PA development (C, L) and caused thickening and overlapping of EOM insertions (F). At 60 hpf EOMs in Dio3 MO knockdowns showed early muscle differentiation (L), but were clustered together and indistinct in wholemount (L) or on sections (N). AM, anterior mandibulae. Scale bar=50 or 100 µm as indicated.

Expression Data

Genes:	EGFP myhz2 myod1
Fish:	WT WT + MO2-thraa mpv17^a9/a9; zf13Tg mpv17^a9/a9; zf13Tg + MO1-dio3b mpv17^a9/a9; zf13Tg + MO2-thraa
Knockdown Reagents:	MO1-dio3b MO2-thraa
Anatomical Terms:	dorsal oblique extraocular muscle dorsal rectus extraocular musculature lateral rectus mandibular arch skeleton muscle medial rectus pharyngeal arch muscle ventral oblique extraocular muscle ventral rectus
Stage Range:	Pec-fin to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-thraa mpv17^a9/a9; zf13Tg + MO2-thraa
Knockdown Reagent:	MO2-thraa
Observed In:	dorsal oblique extraocular muscle dorsal rectus extraocular musculature lateral rectus medial rectus pharyngeal arch muscle ventral oblique extraocular muscle ventral rectus
Stage Range:	Pec-fin to Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 373(2), Bohnsack, B.L., and Kahana, A., Thyroid hormone and retinoic acid interact to regulate zebrafish craniofacial neural crest development, 300-309, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.