FIGURE

Fig. 2

ID

ZDB-FIG-130228-35

Publication

Parente et al., 2013 - Hypoxia/Reoxygenation cardiac injury and regeneration in zebrafish adult heart

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Fig. 2

Inflammatory response induced by H/R in vivo.

Representative confocal microscopy images (a-h) showing neutrophils (yellow fluorescence or green fluorescence) and macrophages (red fluorescence) infiltration in double transgenic line Tg(MPO:EGFP)×Tg(LysC:DsRed) in control (C) and at different time points (4 h, 6 h, and 14 h) after H/R. Neutrophils are either yellow (LysC⁺/MPO⁺) or green (predominantly, MPO⁺) cells (arrows in the 6 h image); red macrophages are LysC⁺ cells (arrow in the 4 h image). Hoechst stains cell nuclei; (a-d) calibration bar = 100 µm, (e-h) calibration bar = 20 µm.The peak inflammatory response occurred at the 6 h time point after H/R This experiment was performed three times with similar results.

Expression Data

Genes:	DsRed2 GFP
Fish:	i114Tg; nz50Tg
Condition:	hypoxia
Anatomical Terms:	macrophage neutrophil
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One