FIGURE

Fig. 5

ID

ZDB-FIG-120814-36

Publication

Mitra et al., 2012 - Requirement for a uroplakin 3a-like protein in the development of zebrafish pronephric tubule epithelial cell function, morphogenesis, and polarity

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Disruption of epithelial polarity in upk3l morphants.

(A–B) Localization of Prkcz (A) or Pard3 (B), Na⁺/K⁺-ATPase, and nuclei in cross-sectioned embryos treated with control (CNT-MO) or upk3l MO (upk3l-MO). (C) Distribution of Na⁺/K⁺-ATPase and nuclei in 2-dpf control or morphant embryos. (D–E) TEM analysis of the proximal segment of PTs from control or morphant embryos. (D) Cross section of tubule show that it is comprised of 5–6 cells surrounding a central lumen. (E) At higher magnification, control lumens were filled with microvilli (mv) and cilia (ci), whereas microvilli were absent in morphants. (F) Immunolocalization of phosphorylated ERM proteins (P-ERM) in frozen sections of embryos. Sections were co-stained for Na⁺/K⁺-ATPase and nuclei. (G) Immunostaining of cilia-associated acetylated-tubulin and nuclei in the PT of laterally oriented embryos. p: proximal tubule, d: distal tubule.

Expression Data

Antibodies:	Ab1-atp1a1 Ab1-ezr Ab1-prkcg Ab1-tuba Ab2-pard3
Fish:	WT WT + MO1-upk3b
Knockdown Reagent:	MO1-upk3b
Anatomical Terms:	epithelial cell apical side epithelial cell apical surface epithelial cell basal surface epithelial cell lateral surface pronephric duct cilium pronephric tubule epithelial cell
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-upk3b
Knockdown Reagent:	MO1-upk3b
Observed In:	epithelial cell microvillus pronephric duct cilium pronephric duct epithelial cell
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One