FIGURE

Fig. 3

ID

ZDB-FIG-120814-34

Publication

Mitra et al., 2012 - Requirement for a uroplakin 3a-like protein in the development of zebrafish pronephric tubule epithelial cell function, morphogenesis, and polarity

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Fig. 3

Pronephric clearance, heart rate, and pericardial area in control and morphant embryos.

(A) Clearance of 70 kDa-TRITC dextran injected into the common cardinal vein of 54-hpf control (top panel) or morphant (bottom panel) embryos. The fluorescence intensity of the sampled area (circled) was measured in each embryo 0, 5, and 24 h following dextran injection. (B) The heart rate, pericardial area, and the dextran retention was recorded for each embryo and the values relative to t = 0 (post dextran injection) were calculated. Data are reported as mean ± SEM (n = 25). (C) Uptake of 500 kDa or 10 kDa FITC-dextran in control or morphant PT epithelial cells. Tubule lumens are marked by asterisks, while internalized dextran is indicated by arrows. Note the incomplete ring of actin in morphant PT cells.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-upk3b y1Tg + MO1-upk3b
Knockdown Reagent:	MO1-upk3b
Observed In:	blood circulation heart contraction pericardium renal filtration
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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