Fig. 3

Leukocyte Depletion Prevents V12RAS⁺ Cell Growth, which Can Be Partially Rescued by dmPGE₂(A–I) Representative images of flank skin regions illustrating numbers of V12RASeGFP⁺ transformed cells after morpholino (Mo) knockdown of pu.1 and gcsfr (for depletion of both neutrophils and macrophages); gcsfr alone (for depletion of neutrophils); irf8 or a lower dose (2 nl/embryo) of pu.1 morpholino alone (for depletion of macrophages) with or without additional treatment with dmPGE_2.(J) Graphic illustration of how dmPGE₂ can rescue the number of transformed cells in morphants in which both neutrophils and macrophages have been specifically depleted (p < 0.01, n = 33, 32, and 34).(K) Graphic illustration of how dmPGE₂ can only partially rescue morpholino knockdowns that largely target neutrophils (p < 0.001, n = 25, 25, and 22).(L and M) Similar graphic illustration of how dmPGE₂ can rescue morpholino knockdowns that largely target macrophages (irf8 Mo-p < 0.05, n = 24, 26, and 23; 2 nl pu.1 Mo-p < 0.05, n = 31, 30, and 34).The scale bar represents 100 µm. See also Figures S3 and S4.