FIGURE

Fig. 7

ID

ZDB-FIG-120720-39

Publication

Huycke et al., 2012 - Hedgehog-dependent proliferation drives modular growth during morphogenesis of a dermal bone

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Fig. 7

Osteoblast stage markers are unaffected in ihha mutants. Confocal projections showing expression of osteoblast markers at 3 and 5 dpf by whole-mount fluorescent in situ hybridization. Wild-type expression is shown in the main panels and ihha mutant expression is shown in the insets. Images are lateral views with dorsal upwards and anterior towards the left. (A,B) At 3 dpf, runx2a/b expression surrounds and overlaps ventral sp7 expression in the Op, and is unchanged in ihha mutants. (C) col10a1 is expressed in the dorsal Op at 3 dpf and is unchanged in ihha mutants. (D,E) At 5 dpf, runx2a/b expression colocalizes with sp7 along the vp edge and is also apparent ventral to the vp edge in non-sp7-expressing cells. Expression is similar in ihha mutants. (F) At 5 dpf, col10a1 is expressed at high levels throughout the Op. The domains of these markers appear slightly reduced at 5 dpf, matching the reduction in Op size. Scale bar: 50 μm.

Expression Data

Genes:	col10a1a runx2a runx2b
Fish:	WT ihha^{hu2131/hu2131}
Anatomical Term:	unspecified
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ihha^{hu2131/hu2131}
Observed In:	osteoblast differentiation
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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