Fig. 4

Analysis of the her1^hu2124/bea ^tm98 double mutant phenotype.

Brightfield images of wild type, bea^tm98 and her1^hu2124/bea^tm98 mutant embryos at the 10–12 somite stage, lateral views, anterior to left. Compared to the wild type embryo (A), the somite borders posterior of the 4^th somite are disrupted in the bea^tm98 mutant (B, asterisks indicate correctly formed somites). All somitic borders are disrupted in the her1^hu2124/bea^tm98 double mutant (C). In situ hybridisation analysis of myoD expression at 10–12 somites (D–F), dorsal views, anterior to top. In line with the morphological phenotypes, half segmental myoD expression is disrupted posterior to the 4^th somite in bea^tm98 (E, asterisks mark residual expression in somites 1–4) and along the entire body axis in her1^hu2124/bea^tm98 double mutants (F) compared to wild type (D). In situ analysis of eplin expression at prim 6 stage (G–J) lateral views, anterior to left. eplin is expressed in v-shape at the somite borders in the wild-type (G). Disturbed eplin expression is observed in the first somites of the her1^hu2124 mutant (H, bracket), posterior to the somite 4 in the bea^tm98 mutant (I, bracket) and in all somites in the double mutant situation (J). (A–F) 10–12 somite stage, (G–J) prim 6 stage.