FIGURE

Fig. 2

ID

ZDB-FIG-120601-101

Publication

Cai et al., 2012 - Cellular retinoic acid-binding proteins are essential for hindbrain patterning and signal robustness in zebrafish

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Fig. 2

crabp2a is RA inducible and required for signal robustness. (A-F) Whole-mount ISH for crabp2a/b expression at 15 hpf; dorsal views, anterior left. crabp2a (A-C) is expressed posterior to the R5/6 boundary, whereas crabp2b (D-F) expression is restricted to somites. Treatment with 10 nM RA expands crabp2a expression anteriorly throughout the CNS (B) but has no effect on crabp2b (E). Treatment with 5 μM DEAB eliminates crabp2a expression (C) but does not disrupt crabp2b (F). (G-R) pax2a, krox20 and hoxb4 expression at 18 hpf in uninjected controls (G-I), Crabp2a-MO-injected (J-L) and crabp2a mRNA-injected embryos (M-R) treated with 1 nM RA (H,K), 10 nM RA (I,L,N,Q) or 10 μM DEAB (O,R). so, somites.

Expression Data

Genes:	crabp2a crabp2b egr2b hoxb4a pax2a
Fish:	WT WT + MO1-crabp2a
Conditions:	chemical treatment: all-trans-retinoic acid chemical treatment: 4-(diethylamino)benzaldehyde
Knockdown Reagent:	MO1-crabp2a
Anatomical Terms:	central nervous system midbrain hindbrain boundary rhombomere 3 rhombomere 5 rhombomere 6 rhombomere 7 rhombomere 8 somite spinal cord neural rod anterior region
Stage Range:	10-13 somites to 14-19 somites

Expression Detail

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Phenotype Data

Fish:	WT WT + MO1-crabp2a
Conditions:	chemical treatment: all-trans-retinoic acid chemical treatment: 4-(diethylamino)benzaldehyde
Knockdown Reagent:	MO1-crabp2a
Observed In:	hindbrain morphogenesis midbrain hindbrain boundary rhombomere 3
Stage Range:	10-13 somites to 14-19 somites

Phenotype Detail

Acknowledgments

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