FIGURE

Fig. 3

ID

ZDB-FIG-120405-78

Publication

Chernyavskaya et al., 2012 - The voltage-gated calcium channel CACNB2 (β2.1) protein is required in the heart for control of cell proliferation and heart tube integrity

Other Figures

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Fig. 3

Quantification of looping using plane of the atrioventricular junction to define the “looping angle.” A: Wild-type Tg(myl7:EGFP HsHRAS) hearts were imaged. The “looping angle” was defined as the angle created between the plane of the cardiac atrioventricular junction and the embryo anteroposterior axis, as diagrammed. A: In wild-type embryos, the average looping angle decreased significantly as development proceeded (P < 0.001; n = 20 embryos/time point). B: At 48 hpf, MO1 or MO2 morphant hearts displayed a significantly greater average looping angle than wild-type (P < 0.01; n = 20 embryos/time point), indicating less looping had occurred. The looping angle was restored to wild-type ranges by co-injecting β2.1cRNA along with MO1 (P = 0.40). avj, atrioventricular junction.

Expression Data

Gene:	EGFP
Fish:	s883Tg s883Tg + MO1-cacnb2a s883Tg + MO2-cacnb2a
Knockdown Reagents:	MO1-cacnb2a MO2-cacnb2a
Anatomical Terms:	atrial myocardium plasma membrane ventricular myocardium plasma membrane
Stage Range:	Prim-15 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s883Tg + MO1-cacnb2a s883Tg + MO2-cacnb2a
Knockdown Reagents:	MO1-cacnb2a MO2-cacnb2a
Observed In:	heart heart looping
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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