FIGURE

Fig. S8

ID

ZDB-FIG-120405-29

Publication

Jao et al., 2012 - A zebrafish model of lethal congenital contracture syndrome 1 reveals Gle1 function in spinal neural precursor survival and motor axon arborization

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Fig. S8

Most gfap:GFP⁺ radial glia are Sox2⁺ neural precursors, which are located close to the apical surface and are mutually exclusive with motoneurons. (A-D) Transverse cryosections through the spinal cord of wild-type embryos harboring the gfap:GFP transgene were stained with anti-Sox2 antibody at 24 hpf. Most, if not all, gfap:GFP⁺ cells (A) are also Sox2⁺ (B, merged channels in C,D). (E-P) Transverse cryosections through the spinal cord of gle1 morphants harboring the mnx1:TagRFP-T transgene were stained with anti-Sox2 antibody at 36 hpf. Note that a portion of mnx1:TagRFP-T⁺ motoneurons (arrowheads) are located in close proximity to some of the Sox2⁺ neural precursors (arrows). However, these two signals do not overlap (merged channels in G,K,O and H,L,P). Nuclei were counterstained by TO-PRO-3. Scale bars: 7.5 μm in D; 10 μm in H,L,P.

Expression Data

Genes:	GFP sox2 TagRFP
Fish:	mi2001Tg vu504Tg
Anatomical Terms:	spinal cord motor neuron spinal cord neuronal stem cell spinal cord radial glial cell
Stage Range:	Prim-5 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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