Fig. S2

gle1^-/- mutants have small eyes and spinal cord. (A) H&E-stained frontal transverse sections through the eyes of wild-type (WT) and gle1^-/- mutant larvae at 4 dpf. All retinal cell layers are present in the gle1^-/- eye, albeit in reduced numbers. Cells with condensed nuclei typical of apoptotic cells can be seen in the gle1^-/- retina, especially in the ciliary marginal zone (red dashed outlines). cmz, ciliary marginal zone; prl, photoreceptor layer; opl, outer plexiform layer; onl, outer nuclear layer; inl, inner nuclear layer; ipl, inner plexiform layer; rgc, retinal ganglion cell layer; le, lens. Images were taken under the same magnification. Scale bar: 100 μm. (B) Quantification of spinal cord size (representative spinal cord sections shown in Fig. 2K,L) by measuring the area of 7-μm transverse plastic sections of the spinal cord at the region above the yolk extension using ImageJ. Data were collected from three randomly selected 5-dpf gle1^-/- mutant larvae and three of their wild-type siblings; six sections per larva were measured. Statistical significance was determined using the unpaired t-test. The spinal cords of the gle1^-/- mutant larvae were approximately 73% of wild-type size.