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Fig. 4

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ZDB-FIG-120329-63

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Roostalu et al., 2012 - In Vivo imaging of molecular interactions at damaged sarcolemma

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Fig. 4

Annexin Kinetics in Myofibers
(A) Comparative relocation kinetics of annexins. Green letters in name or pie graph sector denote annexin tagged with mTFP1, and magenta letters indicate fusion to mOrange1. The pie graph illustrates relative intensity of the two fluorophores in the lesion. Anxa6 and Anxa11a are the fastest to accumulate in the wound, followed by Anxa2a and Anxa1a.
(B) Kinetics of mTFP1-tagged annexins at the site of the lesion (mean from eight experiments). Anxa6-mTFP1 showed a rapid increase in the lesion, whereas a time delay was observed for other annexins.
(C) FRAP was carried out with annexins tagged with mOrange1 (mean of 25 experiments). The motility of different annexins in the cytoplasm is comparable.
(D) Domain architecture of zebrafish annexins. Green boxes denote Ca²⁺-sensitive domains. Sizes are proportional to sequence length.
(E) Accumulation of mutant Anxa6, missing the 4 C-terminal Ca²⁺-sensing domains, is impaired in comparison with full-length Anxa6; compare with the Anxa6 panel in (B).Scale bars represent 4 μm. See also Figure S4.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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Reprinted from Developmental Cell, 22(3), Roostalu, U., and Strähle, U., In Vivo imaging of molecular interactions at damaged sarcolemma, 515-529, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell