Fig. S4
- ID
- ZDB-FIG-120201-20
- Publication
- Dworkin et al., 2012 - Midbrain-hindbrain boundary patterning and morphogenesis are regulated by diverse grainy head-like 2-dependent pathways
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Expression of non-MHB developmental markers is unaffected in grhl2b-morphants. (A-N′) grhl2b morphants were examined for potential differences in expression of markers of the MHB at 9-10 hpf (A,A′, wnt1; B,B′, her5; C,C′, pax2a), 14 hpf (D,D′; wnt1, inset, dorsal view) and 23h pf (E-E2 wnt1, inset, dorsal view); margin and neural plate at 7-10h pf (F,F2, fgf8; G,G′, dlx3), neural crest at 8 hpf (H,H′, snail2), notochord at 8 hpf I,I2, hatching gland 1 (hgg); J,J′, goosecoid (gsc); K,K2 no tail (ntl) and ectoderm-derived tissues at 18 hpf, such as primitive neurons L,L′, islet 1 (isl1), neural crest (M,M′, sox9b) and floor plate of the neural tube N,N2, sonic hedgehog (shh). A-C show dorsal views, anterior towards top. D,E,L-N show lateral views, anterior towards left. F,H-K show dorsal views, anterior towards left. G shows a lateral view, anterior towards the top. Subtle differences in staining patterns were occasionally observed as a secondary, non-specific consequence of gross morphant morphology. |