FIGURE

Fig. 7

ID

ZDB-FIG-111215-40

Publication

Phillips et al., 2011 - Harmonin (Ush1c) is required in zebrafish Muller glial cells for photoreceptor synaptic development and function

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Synaptic maturation is impaired in ush1c morphants. (A,B) Anti-SV2 (red) and -GluR2/3 (green) antibodies mark the pre-and postsynaptic regions of the OPL, respectively. Tightly associated localization of these factors is apparent in the control retina (A), and is notably disrupted in the morphant retina (B) at 5 dpf. (C–F) Electron micrographs of cone pedicles in 6 dpf control and morphant larvae. In control retinas (C,E), the presence of multiple triads (boxed areas), consisting of a synaptic ribbon (r), arciform density (arrow) and postsynaptic processes from bipolar (b) and horizontal (h) cells, indicate normal synaptic maturation. An enlargement of a triad is shown in E. In morphant retinas (D,F), fewer triads are present (boxed areas in D; enlarged in panel F), and floating ribbons (r) and distant postsynaptic processes (h) are observed in cone pedicles. Scale bars: 20 μm (A,B); 1 μm (C,D); 500 nm (E,F).

Expression Data

Antibodies:	Ab-SV2 Ab1-grm
Fish:	AB AB + MO1-ush1c
Knockdown Reagent:	MO1-ush1c
Anatomical Terms:	retinal outer plexiform layer axon terminus retinal outer plexiform layer dendritic spine
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-ush1c
Knockdown Reagent:	MO1-ush1c
Observed In:	postsynaptic density assembly retinal cone cell cytoskeleton of presynaptic active zone retinal outer plexiform layer postsynaptic density retinal outer plexiform layer synapse synapse assembly
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.