FIGURE

Fig. S3

ID

ZDB-FIG-110922-12

Publication

Pyati et al., 2011 - p63 Mediates an Apoptotic Response to Pharmacological and Disease-Related ER Stress in the Developing Epidermis

Other Figures

All Figure Page

Back to All Figure Page

Fig. S3

related to Figure 4: Brefeldin A treatment increases puma expression and ER-stress induced apoptosis in zebrafish embryos.

Brefeldin A (5μg/ml) treatment causes increased puma expression in the lens and tail (B) regions compared to ethanol treated zebrafish embryos (A). In Brefeldin A treated embryos, puma knockdown strongly blocks the 4-hour apoptotic response in the lateral tail epidermis (puma mo) as compared to embryos treated with ethanol (cntrl etoh) or embryos injected with a control morpholino (ctrl mo) (C). The apoptotic response was analyzed by AO staining. Cropped photos of zebrafish tail regions were quantitated for total fluorescence intensity with Volocity software, and values were normalized to controls. (Black asterisk shows the epiphysis; white arrowhead shows the lens)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 21(3), Pyati, U.J., Gjini, E., Carbonneau, S., Lee, J.S., Guo, F., Jette, C.A., Kelsell, D.P., and Look, A.T., p63 Mediates an Apoptotic Response to Pharmacological and Disease-Related ER Stress in the Developing Epidermis, 492-505, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell