FIGURE

Fig. 5

ID

ZDB-FIG-110920-73

Publication

Eames et al., 2011 - Mutations in fam20b and xylt1 Reveal That Cartilage Matrix Controls Timing of Endochondral Ossification by Inhibiting Chondrocyte Maturation

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Fig. 5

Reduction of Alcian blue staining in fam20b and xylt1 mutants is less severe than in uxs1 mutants.

A–E, Alcian blue-stained 6 dpf ceratohyals. Compared to wild types (A), Alcian blue staining appeared lower in fam20b^b1127 (B) and xylt1^b1128 (C) mutants. Staining in fam20b^b1127;xylt1^b1128 double mutants (D) was decreased in comparison to single mutants, although uxs1 mutants (E) showed the greatest decrease in Alcian blue staining. F, Quantitation of Alcian blue in lysates of 6 dpf larvae revealed statistically significant changes (ANOVA p<0.0001) for each mutant class. Compared to wild-type Alcian blue staining, fam20b^b1127 mutants had 50±3.0%, xylt1^b1128 mutants had 57±2.0%, fam20b^b1127;xylt1^b1128 double mutants had 39±2.6%, and uxs1 mutants had 22±1.5%. G, Simplified pathway of PG synthesis, demonstrating that uxs1 is upstream of both xylt1, which adds xylose to a serine (Ser) residue of the core protein, and also fam20b, which phosphorylates (P) xylose in the nascent GAG chain. Abbreviations: CSPGs = chondroitin sulfate proteoglycans; HSPGs = heparan sulfate proteoglycans.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fam20b^b1127/b1127 (AB) uxs1^w60/w60 xylt1^b1128/b1128 (AB) fam20b^b1127/b1127; xylt1^b1128/b1128 (AB)
Observed In:	ceratohyal cartilage
Stage:	Day 6

Phenotype Detail

Acknowledgments

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