FIGURE

Fig. S1

ID

ZDB-FIG-110920-44

Publication

McGraw et al., 2011 - Lef1 is required for progenitor cell identity in the zebrafish lateral line primordium

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Fig. S1

lef1^nl2 mutants lack terminal NMs and have a truncated pLL nerve. (A,B) Wild-type sibling and lef1^nl2 mutant larvae at 4 dpf. The pLL nerve is visualized by expression of the TgBAC(neurod:EGFP) transgene. NMs are labeled with DASPEI. (A) Wild-type embryo contains NMs L1-L5, a secondary (2°) NM and the terminal cluster (tc). (B) lef1^nl2 mutant contains NMs L1-L5 and a 2° NM, but lacks a terminal cluster (tc). In addition, the lef1^nl2 mutant has a truncated pLL nerve (white arrow). (C,D) Functional hair cells in the final deposited NM labeled with FM 1-43 (red) in wild-type (C) and lef1^nl2 larvae (D) at 4 dpf. Nuclei are labeled with DAPI. (E) Quantification of FM 1-43-labeled hair cells showing no significant difference between wild-type and mutant larvae (F) Average number of DAPI-labeled nuclei per NM in wild-type and lef1^nl2 (n=6, mean ± s.e.m.). Scale bar: 20 μm.

Expression Data

Gene:	EGFP
Fish:	nl1Tg lef1^nl2/nl2; nl1Tg
Anatomical Term:	posterior lateral line nerve
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lef1^nl2/nl2; nl1Tg
Observed In:	posterior lateral line nerve posterior lateral line neuromast posterior-most region
Stage:	Day 4

Phenotype Detail

Acknowledgments

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