Fig. 8

Activation of Notch signaling does not restore Calb2b⁺ cell formation in pharyngeal epithelium after miR-200 knock-down. Experimental conditions are indicated in the top right-hand corner, embryonic stage and scale bar are in the bottom left-hand corner and marker expression is in the bottom right-hand corner or in attached insets. Anterior is towards the top. Ventral views. Scale bars: 10 μm. (A,D,G) Confocal projections of grafted endodermal (Tar^*) cells (in wild type) expressing triple MOmiR-200 (green) and Calb2b (red/yellow). The arrow indicates a severe reduction in Calb2b⁺ cell number in the triple MOmiR-200-expressing branchial arch epithelium (b1). (B,E,H) Confocal projections of grafted endodermal (Tar^*) cells (in wild type) expressing HsNicd (red) and Calb2b (blue/purple). Arrowheads indicate Calb2b⁺ cells in the HsNicd hyoid epithelium (h). (C,F,I) Confocal projections of grafted endodermal (Tar^*) cells (in wild type) co-expressing HsNicd, triple MOmiR-200 (red) and Calb2b (blue). The arrow indicates the absence of Calb2b⁺ cells in the HsNicd and triple MOmiR-200-expressing (red) hyoid epithelium (h).