Fig. 7
- ID
- ZDB-FIG-110719-5
- Publication
- Sorokina et al., 2011 - MIP/Aquaporin 0 Represents a Direct Transcriptional Target of PITX3 in the Developing Lens
- Other Figures
- All Figure Page
- Back to All Figure Page
Analysis of mip1 expression in pitx3-mo and control embryos via in situ hybridization and RT-PCR. A, D, F-H. Normal mip1 expression in control-injected embryos at 29-, 34- and 48-hpf. B, C, E, I–K. Altered mip1 expression is observed in pitx3 morphants at 29-hpf with 64% of embryos demonstrating a complete absence of mip1 expression (B) and the remaining larvae showing markedly reduced mip1 expression (C and I). Reduced mip1 expression is also observed in 34- and 48-hpf embryos (E, J, K). Red arrows show sites of expected mip1 expression. Scale bars: A–E: 100 μM; F–L: 20 μM. L. Results of semi-quantitative RT-PCR showing reduced expression of mip1 in pitx3 morphants at early stages of development (red arrow). |
| Gene: | |
|---|---|
| Fish: | |
| Knockdown Reagent: | |
| Anatomical Terms: | |
| Stage Range: | 20-25 somites to Protruding-mouth |
| Gene | Antibody | Fish | Conditions | Stage | Qualifier | Anatomy | Assay |
|---|---|---|---|---|---|---|---|
| mipa | WT | control | Prim-5 | ISH | |||
| mipa | WT | control | Prim-15 | ISH | |||
| mipa | WT | control | Long-pec | ISH | |||
| mipa | WT | standard conditions | 20-25 somites | Not Detected | RTPCR | ||
| mipa | WT | standard conditions | 26+ somites | RTPCR | |||
| mipa | WT | standard conditions | Prim-5 | RTPCR | |||
| mipa | WT | standard conditions | Prim-15 | RTPCR | |||
| mipa | WT | standard conditions | Protruding-mouth | RTPCR | |||
| mipa | WT + MO3-pitx3 | standard conditions | 20-25 somites | Not Detected | RTPCR | ||
| mipa | WT + MO3-pitx3 | standard conditions | 26+ somites | Not Detected | RTPCR |