FIGURE

Fig. 8

ID

ZDB-FIG-110712-58

Publication

Sedletcaia et al., 2011 - Heart chamber size in zebrafish is regulated redundantly by duplicated tbx2 genes

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Fig. 8

Cardiomyocyte proliferation is decreased in the tbx2ab morphants. Embryos were pulse-labeled with bromodeoxyuridine (BrdU), and after being fixed, were co-stained to detect BrdU+ cells (green) and MF20+ cardiomyocytes (red). Hearts were imaged, and the yellow (double positive) cells counted (examples indicated by the small arrows). A,B: The top panels show representative wild-type (A) and morphant (B) embryos at <32 hpf. C: The lower panel shows quantification of the average BrdU+ cardiomyocytes, in each case from several randomly chosen embryos, n = 5. The ** indicates statistical significance compared with wild-type, according to Student′s t-test, P < 0.02. This experiment was repeated several times and reproducibly showed significantly decreased relative levels of BrdU+ cardiomyocytes in morphants, although the actual number of double-labeled cells varies depending on the efficiency of BrdU labeling.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TU + MO1-tbx2
Knockdown Reagent:	MO1-tbx2
Observed In:	cardiac muscle cell proliferation
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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