FIGURE

Fig. 1

ID

ZDB-FIG-110712-49

Publication

Sedletcaia et al., 2011 - Heart chamber size in zebrafish is regulated redundantly by duplicated tbx2 genes

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Fig. 1

Both tbx2a and tbx2b are expressed in the primitive heart tube and transcripts become restricted to the atrioventricular canal (AVC) by 2 days postfertilization (dpf). A–D,G–I: Shown are representative embryos (n > 30 for each) analyzed by in situ hybridization to detect transcripts for tbx2a (A,B,G), tbx2b (C,D,H), or the AVC marker bmp4 (I). A–D: In addition to strong staining in the head, transcripts are readily detected in the primitive heart tube at 36 hpf (A,C, between the dashed lines) and subsequently in the AVC at 3 dpf (B,D, arrowheads marking the cushion tissue on either side of the AVC). E,F: Also shown for purpose of orientation are similarly staged myl7:gfp transgenic embryos that show the position of the heart tube at 36 hpf (E) and the AVC at 3 dpf (F). G–I: The restriction to the AVC can be seen starting already at 2 dpf, compared with bmp4. Control sense strand probes failed to generate any detectable signal under the identical conditions (not shown).

Expression Data

Genes:	bmp4 EGFP tbx2a tbx2b
Fish:	AB/TU twu26Tg
Anatomical Terms:	atrioventricular canal heart heart tube
Stage Range:	Prim-25 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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