FIGURE

Fig. 8

ID

ZDB-FIG-110324-32

Publication

Sheets et al., 2011 - Ribeye is required for presynaptic CaV1.3a channel localization and afferent innervation of sensory hair cells

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Fig. 8

Ribeye depletion disrupts clustering of presynaptic Ca_V1.3a channels in hair cells. (A-D) Ribeye b (green) and Ca_V1.3a (red) immunolabel in cross-sections of anterior lateral line neuromasts at 4 dpf. Merged images include DAPI (blue). Scale bar: 3 μm. (A) Ca_V1.3a clusters are colocalized with Ribeye b-labeled punctae in control MO-injected larvae. (B) Ca_V1.3a clusters are absent in ribeye a & b double morphants (50 μM and 750 μM, respectively). (C) Ca_V1.3a clusters are absent in ribeye b MO-injected (1 mM) larvae. (D) Ca_V1.3a clusters in ribeye b MO-injected O (500 μM) larvae. Ca_V1.3a colocalized with the larger Ribeye b punctae in a neuromast with weak Ribeye b knockdown. (E) RT-PCR analysis of tails from 4 dpf larvae injected as in A and B. gapdh was used as a loading control and pcdh15 was used as a hair-cell specific transcript control.

Expression Data

Genes:	cacna1da ctbp2l
Antibodies:	Ab1-cacna1da Ab3-ctbp2l
Fish:	WT WT + MO2-ctbp2l WT + MO1-ctbp2a + MO2-ctbp2l
Knockdown Reagents:	MO1-ctbp2a MO2-ctbp2l
Anatomical Terms:	anterior lateral line neuromast neuromast hair cell neuromast hair cell presynaptic active zone posterior lateral line neuromast neuromast hair cell
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-ctbp2l WT + MO1-ctbp2a + MO2-ctbp2l
Knockdown Reagents:	MO1-ctbp2a MO2-ctbp2l
Observed In:	clustering of voltage-gated calcium channels neuromast hair cell voltage-gated calcium channel complex presynaptic membrane assembly
Stage:	Day 4

Phenotype Detail

Acknowledgments

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