FIGURE

Fig. s3

ID

ZDB-FIG-110322-22

Publication

Laux et al., 2011 - Dynamic analysis of BMP-responsive smad activity in live zebrafish embryos

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Fig. s3

Supporting Information Figure 3. smad5 knockdown down-regulates bre:egfp transgene expression primarily in somites. Embryos were injected with 2 ng standard control morpholino (a, d, f), 2 ng smad5 morpholino (b, e, g), or 4 ng smad5 morpholino (c) at the one- to two-cell stage and imaged at 1 dpf. In all images, green indicates bre-driven EGFP expression. Brightfield (a, b, c) and fluorescent (d, e) images, lateral view, anterior left, 80× magnification. f, g: 2D projections of confocal Z-series showing somites, EGFP, and transmission overlays, 400× magnification. Lateral views, anterior left.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-smad5 pt510Tg + MO3-smad5
Knockdown Reagent:	MO3-smad5
Observed In:	cloacal chamber post-vent region retina dorsal region somite whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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