ZFIN Figure: Laux et al., 2011, Fig. 1

Fig. 1

Developmental profile of pSmad1/5/8-mediated transcriptional activity in Tg(bre:egfp)^pt510 embryos. In all images, green represents bre-driven EGFP expression. Magenta represents endothelial expression of Tg(fli1ep:dsRedEx)^um13 or myocardial expression of Tg(-5.1myl7:nDsRed2)^f2, as noted below. g-r, h2, h3, l32: Fluorescence/transmission overlays. a-f: Macro images. a: Six somites. Arrows point to tailbud. b: Twelve somites. Arrow points to myeloid progenitors. c: Eighteen somites. Asterisks denote eyes; arrows point to myeloid cells. d-f: Embryos at 1 (d), 2 (e), and 4 (f) dpf. Lettered arrows denote expression domains highlighted in correspondingly lettered panels below. g-w: 2D projections of confocal Z-series, except h2, h3, i3, and l3, which represent single optical sections extracted from the corresponding Z-series. Magnification, 400×. g-g3: Heart at 1 dpf. Asterisk denotes eye. g2 and g′′ are matched substacks showing EGFP/fli1ep:dsRedEx (overlay) and fli1ep:dsRedEx expression, respectively. Arrows denote endocardium. h-h3: Heart at 2 dpf. h2 and h′′ are matched optical sections of EGFP/ fli1ep:dsRedEx/-5.1myl7:nDsRed2 (overlay) and fli1ep:dsRedEx/-5.1myl7:nDsRed2. Arrows denote endocardium; arrowheads denote myocardium. i-i3: Heart at 4 dpf. i2 and i′′ show overlays of EGFP with fli1ep:dsRedEx, 2D projection and single optical section, respectively. j-j2: Pharyngeal arches, 1 dpf. Asterisk denotes eye. k,k2: Pharyngeal arches, 2 dpf. l-l32: Pharyngeal arches, 4 dpf. l2, l3: Overlays of EGFP with fli1ep:dsRedEx, 2D projection, and single optical section, respectively. l32: Substack of image shown in l. m-m3: Dorsal retina (m2) and trigeminal ganglion (m′′), 1 dpf. n: Pineal gland, 1 dpf. o: Hypothalamus, 1 dpf. p, p2: Ventral mesenchyme and cloaca (arrow), 2 dpf. q, q2: Cloaca, 4 dpf. Arrows delineate cloacal opening. r, r2: Somites, 1 dpf. s: Stomodeum, 2 dpf. Asterisk, presumptive mouth opening; arrows, maxillary process; arrowhead, mandibular process. t: Stomodeum, 4 dpf. Asterisk, open mouth; arrows, maxillary process; arrowhead, mandibular process. u: Pectoral fin bud, 2 dpf. v: Mesenchymal cells of the median finfold (arrows), 2 dpf. w: Spinal cord neurons (arrows), 2 dpf. Spinal cord is bracketed. a: Dorsoposterior view, posterior down. b, d-g, j, k, m, p-r, u-w: Lateral view, anterior left, dorsal up. c: Dorsal view, anterior left. h, i, l: Ventral view, anterior left, left up. n: Dorsal view, left to the right. o: Frontal view, left to the right. s, t: Frontal view, left up. a, atrium; v, ventricle; y, yolk.

Expression Data

Genes:	DsRed2 DsRedx EGFP
Fish:	pt510Tg um13Tg f2Tg; pt510Tg
Anatomical Terms:	atrial endocardium cloacal chamber common myeloid progenitor endocardium epiphysis heart tube hypothalamus maxilla bony projection mesenchyme median fin fold myeloid cell myocardium optic cup dorsal region pectoral fin bud pharyngeal arch somite spinal cord neuron stomodeum tail bud trigeminal ganglion ventral mandibular arch bony projection ventral mesenchyme ventricular endocardium
Stage Range:	5-9 somites to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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