FIGURE

Fig. S2

ID

ZDB-FIG-110316-3

Publication

Segalen et al., 2010 - The Fz-Dsh Planar Cell Polarity Pathway Induces Oriented Cell Division via Mud/NuMA in Drosophila and Zebrafish

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Fig. S2

NuMA is expressed during zebrafish gastrulation.
A-F: Expression of NuMA. In situ hybridization for the NuMA sense probe (A-C) or anti-sense probe (D-F) at the sphere (A, D), shield (B, E) and 75% epiboly (C, F) stages. Animal pole to the top, (C, F) dorsal to the right.
G-H″: The efficiency of the NuMA MO was controlled by showing that the expression of a GFP gene harboring the NuMA MO sequence was abrogated by NuMA MO injection (G-G″) but not by the NuMA control MO (H-H″) in zebrafish embryo injected at the 1 cell stage. GFP channel (G and H), bright field image (G′ and H′) and merge (G″ and H″).
I-J″: Dividing epiblast cells in control (I-I″) and NuMA MO injected embryos (J-J″) stained for α-tubulin (I, I″, J and J″, green), γ-tubulin (I′, I″, J′ and J″, red) and DNA (Hoechst, I″ and J″, blue).

Expression Data

Gene:	numa1
Fish:	WT
Anatomical Terms:	blastodisc epiblast
Stage Range:	Sphere to 75%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 19(5), Segalen, M., Johnston, C.A., Martin, C.A., Dumortier, J.G., Prehoda, K.E., David, N.B., Doe, C.Q., and Bellaiche, Y., The Fz-Dsh Planar Cell Polarity Pathway Induces Oriented Cell Division via Mud/NuMA in Drosophila and Zebrafish, 740-752, Copyright (2010) with permission from Elsevier. Full text @ Dev. Cell