Additional expression data for zebrafish embryonic hepatocytes and zebrafish adult tissues. A. Quantitative real-time RT-PCR to assess transcript levels of LFABP (liver fatty acid binding protein) relative to β-actin in hepatocytes sorted from pools of 80–100 transgenic zebrafish embryos at 72 hpf. N = 2 pools per group. Data shown are means +/- SE. * indicates p<0.05 compared to unsorted. B. Semiquantitative RT-PCR for hepcidin, RGMa, RGMb, hjv, RGMd, and neogenin performed with RNA from adult zebrafish liver and skeletal muscle. Hepcidin expression was detected in the adult liver, but not in adult skeletal muscle. All RGM genes and neogenin were detected in the adult liver and skeletal muscle.
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