FIGURE

Fig. 2

ID

ZDB-FIG-101206-23

Publication

Seth et al., 2010 - Core fucosylation is required for midline patterning during zebrafish development

Other Figures

(all 10)

All Figure Page

Back to All Figure Page

Fig. 2

Knockdown of zfFucT8 leads to early midline defects. A: Ventral views of 48-hpf morphant embryos showing mild cyclopia (i.e., close-set eyes), and a smaller forebrain (arrow). B: Forty-eight-hpf FucT8 morphants have a curved body axis with U-shaped somites (inset), as well as (C) “swelling” in the hindbrain ventricle (arrowhead). D: AAL lectin stains α1,6 fucosyl residues in the eye, which is reduced to background levels in morpholino-injected embryos as compared to control-injected, or no lectin, controls. More than 500 embryos were injected with FucT8 MOs, of which 85-90% demonstrated the mutant phenotype.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-fut8a
Knockdown Reagent:	MO1-fut8a
Observed In:	eye forebrain fourth ventricle somite whole organism
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.