Fig. 5

Eve1 rescues posterior neural development in ich^-/- mutants. Homozygous ich^-/- embryos were injected with eve1 mRNA. Genotype is indicated at the bottom left of each panel, injections at the bottom right, and in situ probes at the top right. Uninjected ich^-/- embryos at 24 hpf (A). Eve1 mRNA injection leads to varying levels of rescue of posterior dorsal axis (C–E). Embryos stained for hoxb1b at 80% epiboly, presumed lateral view, dorsal to the right. Expression of hoxb1b is absent in uninjected ich^-/- embryos (C, D) but is rescued by injection of eve1 mRNA (E). In situ hybridization of wild-type and ich^-/- embryos at 24 hpf (anterior to the left) (F-Q). Neural gene expression and posterior dorsal axis formation was partially rescued by the injection of eve1 mRNA. Rescue of pax2a appears to extend to the midbrain–hindbrain boundary (L-N, arrowheads), whereas egr2b expression appears to extend to rhombomere 5 (O–Q, arrowheads).