FIGURE

Fig. S3

ID

ZDB-FIG-101117-38

Publication

Koide et al., 2009 - Olfactory neural circuitry for attraction to amino acids revealed by transposon-mediated gene trap approach in zebrafish

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Fig. S3

Differential GFP expression in distinct types of OSNs in 2 transgenic fish lines, SAGFF27A (A–D) and OMP:Gal4FF (E–H). Adult OE sections were triple-labeled with antibodies against GFP (A and E; green in D and H), calretinin (B and F; red in D and H), and Gα_olf/s (C and G; blue in D and H). In the SAGFF27A line, GFP is expressed in short dendrite-bearing and calretinin-positive OSNs in the apical layer of the OE (A, B, and D), suggesting that Gal4FF/GFP-expressing cells are the microvillous OSNs. In contrast, in the OMP:Gal4FF fish, GFP is observed in long dendrite-bearing OSNs, the cell bodies of which are located in the basal layer of the OE (E, G, and H). Arrows indicate the dendritic knobs that are positive for both GFP and Gα_olf/s on the epithelial surface. These results indicate that Gal4FF/GFP-expressing cells in the OMP:Gal4FF fish are the ciliated OSNs. (Scale bar: 10 μm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA