Fig. 2

Conserved patterns of differential glomerular innervation of GFP-positive olfactory axons in 3 gene trap lines in adult zebrafish: (A1–A9) SAGFF27A, (B1–B9) SAGFF91B, and (C1–C9) SAGFF179A. (A1–C3) Whole-mount OBs with GFP fluorescence viewed from dorsal (A1–C1), ventral (A2–C2), and lateral (A3–C3) sides. (A4–C8) Horizontal sections of OBs triple-labeled with antibodies against GFP (green), PCAM (red), and SV2 (blue). For individual lines, 5 sections are shown from the dorsal-most (A4–C4) to ventral-most (A8–C8) levels. (A9–C9) Schematic diagrams illustrating different patterns of glomerular innervation by GAL4FF-expressing OSNs in the adult gene trap lines. In SAGFF27A, GFP-positive olfactory axons innervate the lateral chain (lc, red), the ventral glomeruli (vG, pink), and 1 of the mediodorsal posterior glomeruli (mdpG2, blue). In SAGFF91B, GFP-positive olfactory axons innervate 2 of the mediodorsal posterior glomeruli (mdpG2 and mdpG3, blue). In SAGFF179A, GFP-positive olfactory axons innervate the mdpG1-3 (blue), the mediodorsal glomerulus (mdG, blue), and the ventroposterior glomerulus (vpG, light blue). (Scale bars: 100 μm.)