FIGURE

Fig. S1

ID

ZDB-FIG-101104-21

Publication

Uribe et al., 2010 - Id2a influences neuron and glia formation in the zebrafish retina by modulating retinoblast cell cycle kinetics

Other Figures

All Figure Page

Back to All Figure Page

Fig. S1

id2b expression and Id2a morpholino (MO) controls. Zebrafish possess two id2 paralogs, id2a and id2b. (A,B) id2b mRNA is not detected in the retina at 24 hpf (A), whereas at 48 hpf small clusters of cells are positive for id2b (B, arrows). (C,D) Id2 is expressed throughout the neuroepithelial retina and lens at 24 hpf (C) and becomes restricted to the dorsal CMZ by 48 hpf (D). (E,F) Id2 protein is not detected at 24 hpf (E) or at 48 hpf (F) in Id2a morphant retinas. (G) Western blot confirmation of Id2 antibody specificity and MO knockdown. Id2 antibody recognizes a specific band at 15 kDa from 48 hpf larval lysates. MO-mediated knockdown of Id2a leads to reductions in the specific -15 kDa band, whereas MO knockdown of Id2b does not.

Expression Data

Gene:	id2b
Antibody:	Ab1-id2
Fish:	WT WT + MO1-id2a WT + MO1-id2b WT + MO1-id2a + MO1-id2b
Knockdown Reagents:	MO1-id2a MO1-id2b
Anatomical Terms:	ciliary marginal zone optic cup retina solid lens vesicle whole organism
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development