FIGURE

Fig. 8

ID

ZDB-FIG-101021-37

Publication

Lyons et al., 2010 - Carboxypeptidase A6 in zebrafish development and implications for VIth cranial nerve pathfinding

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Fig. 8

Knockdown of CPA6 gene function.

(A) Morpholinos were designed to interfere with normal CPA6 mRNA splicing (Ctr-MO: control morpholino; MO-2 and MO-3: CPA6 specific morpholinos; lines: MO binding sites; arrows: primer binding sites). (B) RT-PCR using RNA extracted from either uninjected or morpholino-injected embryos (3 ng) showed CPA6-specific knockdown. (C) 48 hpf embryos injected with control morpholino and CPA6 MO-2. CPA6 morphants exhibited yolk-sac edema. (D) DiI injected into the lateral rectus muscle (LR) of 8 dpf Ctr-MO and MO-2 CPA6-morphant zebrafish larvae retrogradely labeled the VIth cranial nerve (nVI). Scale bar = 40 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-cpa6
Knockdown Reagent:	MO1-cpa6
Observed In:	abducens nerve development
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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