FIGURE

Fig. 2

ID

ZDB-FIG-101021-20

Publication

Kwon et al., 2010 - Identification of early requirements for preplacodal ectoderm and sensory organ development

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Fig. 2

Distinct responses of neural crest and preplacodal ectoderm to graded impairment of Bmp.

(A) Lateral views of foxd3 expression at 11 hpf with anterior up and dorsal to the right. Embryos were treated with indicated concentrations of DM added at 4 hpf. Where indicated the DM concentration was increased to 200 µM (complete Bmp-inhibition) at 5 hpf, 6 hpf or 7 hpf. (B) Lateral views of six4.1 expression at 11 hpf in embryos treated with indicated concentrations of DM beginning at 4 hpf. Treatment with 25 µM DM yields two discrete responses, one in which six4.1 remains confined to two bilateral stripes flanking the neural plate and the other in which six4.1 expression is lost. (C) Lateral views showing expression of preplacodal competence factors tfap2a, tfap2c, foxi1 and gata3 in embryos were treated with 50 µM DM beginning at 4 hpf. Note that tfap2a/c remain broadly expressed in ventral ectoderm whereas foxi1 and gata3 are nearly eliminated.

Expression Data

Genes:	foxd3 foxi1 gata3 six4b tfap2a tfap2c
Fish:	WT
Condition:	chemical treatment: dorsomorphin
Anatomical Terms:	ectoderm neural crest
Stage Range:	75%-epiboly to 1-4 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	chemical treatment: dorsomorphin
Observed In:	cranial neural crest neural crest
Stage:	1-4 somites

Phenotype Detail

Acknowledgments

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