FIGURE

Fig. 2

ID

ZDB-FIG-100830-3

Publication

Hsu et al., 2010 - Multiple domains in the Crumbs Homolog 2a (Crb2a) protein are required for regulating rod photoreceptor size

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Fig. 2

Localization of Crb2a constructs lacking the extracellular domain in rod photoreceptors and transgenic rod morphology. (A) Crb2a constructs lacking the extracellular domain. All constructs have a signal peptide (SP) followed by an HA tag. Crb2a^IntraWTcontains the transmembrane domain and intracellular sequence of Crb2a. Crb2a^IntraΔFBDbears two mutations (Y10A and E16A) within the FERM-binding domain (FBD). Crb2a^IntraΔPBDhas the sequence following the FBD that contains the PDZ-binding domain (PBD) deleted. Crb2a^{IntraΔFBDΔPBD}bears Y10A and E16A mutations within the FBD and the PBD the sequence following the FBD that contains the PDZ-binding domain (PBD) deleted. (B-F″) Confocal z-projections of 6 d rods labeled with GFP (green), Rhodopsin (blue) and anti-HA (red) antibodies. (B) Wild-type rods. (C-C″) Crb2a^IntraWTtransgenic rods. (D-D″) Crb2a^IntraΔFBDtransgenic rods. (E-E″) Crb2a^IntraΔPBDtransgenic rods. (F-F″) Crb2a^{IntraΔFBDΔPBD}transgenic rods. Scale bar, 5 μm (B-F″).

Expression Data

Gene:	EGFP
Antibody:	Ab4-rho
Fish:	slc45a2^b4/b4; fl1Tg slc45a2^b4/b4; fl1Tg; umz10Tg slc45a2^b4/b4; fl1Tg; umz13Tg slc45a2^b4/b4; fl1Tg; umz16Tg slc45a2^b4/b4; fl1Tg; umz19Tg
Anatomical Term:	retinal rod cell
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Open Access.

Full text @ BMC Cell Biol.