FIGURE

Fig. S3

ID

ZDB-FIG-100830-12

Publication

Hsu et al., 2010 - Multiple domains in the Crumbs Homolog 2a (Crb2a) protein are required for regulating rod photoreceptor size

Other Figures

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Fig. S3

The Golgi apparatus, labeled by antibodies to Golgin subfamily A member 5 (GOLGA5), in 6 d wild-type and Crb2a transgenics. (A-G″′) Confocal z-projection of GFP-expressing rods in sections of 6 d retinas labeled with anti-GOLGA5 antibodies (red) and anti-HA (blue). (A, A′) Wild-type rods. (B-B″′) Crb2a^FLtransgenic rods. (C-C″′) Crb2a^Extra_TMtransgenic rods. (D-D″′) Crb2a^Extra_Secrtransgenic rods. (E-E″′) Crb2a^IntraWTtransgenic rods. (F-F″′) Crb2a^IntraΔFBDtransgenic rods. (G-G″′) Crb2a^IntraΔPBDtransgenic rods. (H) Western blot of 5 d wild-type zebrafish labeled with anti-GOLGA5 antibodies reveals a single protein of the expected molecular weight. The western blot was performed as described in [8], anti-GOLGA5 (Sigma HPA000992) was used at 1:1000 and HRP-conjugated goat anti-rabbit was used at 1:30,000. Scale bar, 5 μm.

Expression Data

Gene:	EGFP
Antibody:	Ab1-golga5
Fish:	slc45a2^b4/b4; fl1Tg slc45a2^b4/b4; fl1Tg; umz1Tg slc45a2^b4/b4; fl1Tg; umz4Tg slc45a2^b4/b4; fl1Tg; umz7Tg slc45a2^b4/b4; fl1Tg; umz10Tg slc45a2^b4/b4; fl1Tg; umz13Tg slc45a2^b4/b4; fl1Tg; umz16Tg slc45a2^b4/b4; fl1Tg; umz19Tg
Anatomical Terms:	retinal rod cell retinal rod cell Golgi apparatus
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Open Access.

Full text @ BMC Cell Biol.