FIGURE

Fig. 6

ID

ZDB-FIG-100816-7

Publication

Fett et al., 2010 - Parkin Is Protective against Proteotoxic Stress in a Transgenic Zebrafish Model

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Transient knockdown of parkin in zebrafish does not lead to gross morphological alterations.

(A) Genomic structure of zebrafish parkin comprising a total of 12 exons. The splice-blocking GT-grip targets the exon-intron junction of exon 2. (B+C) Verification of the parkin knockdown efficiency by semi-quantitative real-time PCR. (B) The TaqMan™ probe targets the exon 2/exon 3 junction and cannot bind in case of incorrect splicing. (C) Parkin knockdown efficiency at day 1, 2 and 3 after injection of the parkin-specific GT-grip in comparison to a control GT-grip. The amount of zebrafish parkin-specific mRNA was normalized to β-actin in parkin GT-grip-injected zebrafish compared to control GT-grip-injected zebrafish. Quantification is based on 4 independent experiments. ***p<0.001 (D) Parkin-deficient zebrafish embryos do not show obvious morphological alterations. Two-day-old parkin knockdown zebrafish embryos are shown in comparison to control-injected embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One