FIGURE

Fig. 4

ID

ZDB-FIG-100806-55

Publication

Bussmann et al., 2010 - Arteries provide essential guidance cues for lymphatic endothelial cells in the zebrafish trunk

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Fig. 4

Intersegmental arteries are required for LEC migration. (A-C) flt1^enh:RFP (A), SAGFF27C;UAS:GFP (B) and merged (C) unilateral confocal images of control embryos at 4.5 dpf showing lymphatic patterning along aISVs in normal blood vasculature. Arrows indicate ISLVs. (D-F) flt1^enh:RFP (D), SAGFF27C;UAS:GFP (E) and merged (F) unilateral confocal images of kdrl morpholino injected embryos at 4.5 dpf showing LEC migration along arteries that cross-connect abnormally at the horizontal myoseptum, and that do not extend into the dorsal half (indicated by yellow asterisk) of the respective somite. Arrows indicate LECs along the surface of an abnormal aISV. Vertical red bars indicate the DA. (G) Model of lymphatic patterning within the zebrafish trunk. Secondary sprouts from the PCV that do not connect to primary intersegmental vessels migrate to the horizontal myoseptum region where they constitute a pool of PLs. PLs will migrate ventrally or dorsally to form the thoracic duct and ISLVs, respectively, and use arteries as their substrate.

Expression Data

Genes:	EGFP RFP
Fish:	map3k22^{nksagff27cGt/+}; hu5333Tg; nkuasgfp1aTg map3k22^{nksagff27cGt/+}; hu5333Tg; nkuasgfp1aTg + MO1-kdrl
Knockdown Reagent:	MO1-kdrl
Anatomical Terms:	artery intersegmental vessel intersegmental lymph vessel lymph vessel endothelium thoracic duct
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	map3k22^{nksagff27cGt/+}; hu5333Tg; nkuasgfp1aTg + MO1-kdrl
Knockdown Reagent:	MO1-kdrl
Observed In:	intersegmental artery intersegmental lymph vessel
Stage:	Day 4

Phenotype Detail

Acknowledgments

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