FIGURE

Fig. 1

ID

ZDB-FIG-100806-52

Publication

Bussmann et al., 2010 - Arteries provide essential guidance cues for lymphatic endothelial cells in the zebrafish trunk

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Fig. 1

Generation and visualization of flt1^enh:RFP and SAGFF27C;UAS:GFP transgenic lines. (A-C) Arterial-restricted reporter expression in the flt1^enh:RFP transgenic line at 5 dpf (A), compared with kdrl:GFP, which labels all blood endothelial cells (B) allows for discrimination between ISVs connected to the dorsal aorta or PCV. (C) Overlay of A,B. (D) Schematic representation of flt1^enh:RFP expression. (E) Lymphatic reporter expression in the SAGFF27C;UAS:GFP transgenic line at 5 dpf, compared with an angiography (F) that labels perfused blood vessels (red in G). (G) Overlay of E,F. (H) Schematic representation of SAGFF27C;UAS:GFP expression. aISV, arterial ISV; DA, dorsal aorta; DLLV, dorsal longitudinal lymphatic vessel; ISLV, intersegmental lymphatic vessel; PCV, posterior cardinal vein; TD, thoracic duct; vISV, venous ISV.

Expression Data

Genes:	EGFP RFP
Fish:	hu5333Tg; s843Tg map3k22^{nksagff27cGt/+}; nkuasgfp1aTg
Anatomical Terms:	artery intersegmental vessel dorsal aorta dorsal longitudinal lymphatic vessel intersegmental lymph vessel lymph vessel endothelium intersegmental vein blood vessel endothelial cell posterior cardinal vein blood vessel endothelial cell thoracic duct
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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