FIGURE

Fig. 8

ID

ZDB-FIG-100616-82

Publication

Znosko et al., 2010 - Overlapping functions of Pea3 ETS transcription factors in FGF signaling during zebrafish development

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Fig. 8

Etv5-ETS domain binds to the Dusp6 promoter. (A) Biotin labeled PCR product. (B) Biotin labeled mutant Pea3B Dusp6 promoter fragment. (C) Pea3B 18 bp fragment used in competition assays. Underlined sequence represent the putative Pea3B binding site (D) EMSA using 60 bp Dusp6 promoter biotin fragment and GST-Etv5:ETS protein. The Etv5-ETS domain bound to the 60 bp Dusp6 promoter containing the Pea3B site (lane 2). This binding was competed with the non-biotin labeled 60 bp Dusp6 promoter (lanes 3–7) and the Pea3B 18 bp fragment (lanes 8–12). A random oligonucleotide sequence did not compete this binding (lanes 13–17). Binding of Etv5-ETS to the Pea3B mutant Dusp6 promoter was not observed (lanes 18–19).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 342(1), Znosko, W.A., Yu, S., Thomas, K., Molina, G.A., Li, C., Tsang, W., Dawid, I.B., Moon, A.M., and Tsang, M., Overlapping functions of Pea3 ETS transcription factors in FGF signaling during zebrafish development, 11-25, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.