FIGURE

Fig. 3

ID

ZDB-FIG-100519-3

Publication

Ng et al., 2002 - The limb identity gene Tbx5 promotes limb initiation by interacting with Wnt2b and Fgf10

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Fig. 3

Zebrafish tbx5 is necessary for fgf10 and fgf8 expression in the pectoral fin budding region. All panels show the dorsal view of zebrafish embryos with anterior to the top. (A-D) Comparison of fgf10 and fgf8 expression patterns in embryos injected with 10 ng of control (A,C) or tbx5 (B and D) morpholino. (A and B) fgf10 expression in the pectoral fin bud region of 26 hpf (A, arrowheads) was not detected in the tbx5-morpholino injected embryo (B, asterisks). Note that expression remains unchanged in other regions of the embryo. (C and D) In 36 hpf tbx5-morpholino-injected embryos, fgf8 expression could not be detected in the region where the pectoral fin buds develop (D, asterisks), as compared to the control embryos (C, arrowheads point to the expression in the apical fold). fgf8 expression in the midbrain-hindbrain boundary remains unaltered in the injected embryos, as noted by the arrows. (E) fgf10 was not detected in the pectoral fin bud region of 24 hpf hst mutants (asterisks), but remained unaltered in other regions.

Expression Data

Genes:	fgf8a fgf10a
Fish:	WT tbx5a^m21/m21 WT + MO3-tbx5a
Knockdown Reagent:	MO3-tbx5a
Anatomical Terms:	apical ectodermal ridge pectoral fin bud midbrain hindbrain boundary pectoral fin bud
Stage Range:	Prim-5 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tbx5a^m21/m21 WT + MO3-tbx5a
Knockdown Reagent:	MO3-tbx5a
Observed In:	pectoral fin bud
Stage Range:	Prim-5 to Prim-25

Phenotype Detail

Acknowledgments

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