Fig. 3

Pdlim7 colocalizes with full-length but not the hearstrings Tbx5. (A) Schematic of wild-type and heartstrings (hst) Tbx5 proteins. Pdlim7 binding region is denoted in wild-type Tbx5. Red asterisk indicates the truncation site of the hst encoded protein. (B–E) Single transfection of myc-Pdlim7 in COS-7 cells. Cells stained with anti-myc antibodies (B), Alexa-488 phalloidin to stain filamentous actin (C), and DAPI nuclear stain (D). (E) Merged image of B–D. (F–I) Single transfection of full-length HA-Tbx5. Cells stained with anti-HA antibodies (F), Alexa-488 phalloidin (G), and DAPI (H). (I) Merged image of F–H. (J–M) Individual transfection of truncated HA-Tbx5 resembling the encoded hst allele. Cells stained for Tbx5 (J), actin (K), and nucleus (L). (M) Merged image of J–L. (N–Q) Cotransfected COS-7 cells stained for Pdlim7 (N), full-length Tbx5 (O), and nucleus (P). (Q) Merged image of N-P. (R-U) Cotransfected cells stained for Pdlim7 (R), truncated Tbx5 (S), and nucleus (T). (U) Merged image of R-T. Scale bar, 20 μm.