FIGURE

Fig. 7

ID

ZDB-FIG-100107-8

Publication

Soyer et al., 2010 - Rfx6 is an Ngn3-dependent winged helix transcription factor required for pancreatic islet cell development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Impaired endocrine cell differentiation, accumulation of islet progenitor cells and failure of insulin-expressing cells clustering in rfx6 morphants in the zebrafish. (A-X) Ventral views of the pancreas area from embryos analysed by WISH, anterior to the left. (A-L) Hormone expression in the control and rfx6 morphants at 30 hpf. (M-X) Pancreatic expression of isl1, sox4b and neuroD at 24 hpf and arx at 30 hpf in the control and rfx6 morphants. (Y-ZII) Lateral views of the pancreas area from 30 hpf embryos analysed by double-fluorescent WISH for sox4b and isl1, anterior to the left, dorsal to the top. Quantifications (right side of figure) represent the number of positive cells per embryo for controls and morphants. Asterisks (*) indicate that the difference between cell number in control and morphants is statistically significant by Student′s t-test (P<0.001). Scale bar: 14 μm in A-L; 18 μm in M-X.

Expression Data

Genes:	arxa gcga ghrl ins isl1a neurod1 sox4b sst2
Fish:	WT WT + MO1-rfx6 WT + MO2-rfx6
Knockdown Reagents:	MO1-rfx6 MO2-rfx6
Anatomical Term:	posterior pancreatic bud
Stage Range:	Prim-5 to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-rfx6 WT + MO2-rfx6
Knockdown Reagents:	MO1-rfx6 MO2-rfx6
Observed In:	endocrine pancreas endocrine cell endocrine pancreas development
Stage Range:	Prim-5 to Prim-15

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development