FIGURE

Fig. 2

ID

ZDB-FIG-091217-67

Publication

Tallafuss et al., 2009 - DeltaA mRNA and protein distribution in the zebrafish nervous system

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Fig. 2

Dla antibody is specific to zebrafish Dla protein. A and B show confocal images of lateral views of two spinal cord segments at 24 hours postfertilization (hpf); anterior to the left, dorsal up. Dla protein is revealed in green, Islet 1/2 (Isl) protein in red. A: Wild-type embryo showing punctate Dla labeling scattered throughout the spinal cord. B: Dla labeling is abolished in homozygous carriers in the viral insertion line dla^hi781Tg (dla-/-). Isl labeling shows an increased number of RB neurons in homozygous dla^hi781Tg embryos. C,D: Polymerase chain reaction (PCR) on genomic DNA extracted from 6 single embryos (e1-e6) and one control wild-type embryo (ctr). Two different primer sets reveal carriers of the viral insertion in the dla locus versus noncarriers. C: In DNA from heterozygous (+/-) carriers and noncarriers (+/+), dla-specific primers amplify a specific product with the expected size of 450 bp. D: A specific product of 270 bp is amplified in heterozygous (+/-) and homozygous (-/-) carriers whereas no product appears in wild-types (+/+). Scale bar = 8 μm in A,B.

Expression Data

Gene:	dla
Antibodies:	Ab1-dla Ab2-isl
Fish:	WT dla^{hi781Tg/hi781Tg}
Anatomical Terms:	Rohon-Beard neuron spinal cord
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dla^{hi781Tg/hi781Tg}
Observed In:	Rohon-Beard neuron
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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