FIGURE

Fig. 1

ID

ZDB-FIG-091016-6

Publication

Ebarasi et al., 2009 - A reverse genetic screen in the zebrafish identifies crb2b as a regulator of the glomerular filtration barrier

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Fig. 1

Glomerular dye filtration assays on morphants. (A) Control morpholino injected 4 dpf larvae show no uptake of the 500 kDa FITC dextran dye into pronephric tubules (circled by white line). Dye is present within the dorsal aorta lumen (asterisk). In the inset, a schematic of a transverse section shows the relative positions of the somites (S), notochord (N), and pronephric tubules (T). (B) In crb2b morphants, the 500 kDa FITC dye is taken up into endosomes in the pronephric tubule epithelial cells (arrowheads). Similar uptake of the 500 kDa FITC dextran tracer is observed in ralgps1(C), rabgef1 (D), rapgef2 (E) morphants, but not in ga17 morphants (F), which tested negative in this assay. Nuclei are labeled with DAPI. Scale bar, 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-crb2b WT + MO1-eif3m WT + MO1-rabgef1 WT + MO1-ralgps1 WT + MO1-rapgef2b
Knockdown Reagents:	MO1-crb2b MO1-eif3m MO1-rabgef1 MO1-ralgps1 MO1-rapgef2b
Observed In:	glomerular filtration pronephric glomerulus
Stage:	Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 334(1), Ebarasi, L., He, L., Hultenby, K., Takemoto, M., Betsholtz, C., Tryggvason, K., and Majumdar, A., A reverse genetic screen in the zebrafish identifies crb2b as a regulator of the glomerular filtration barrier, 1-9, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.