FIGURE

Fig. 7

ID

ZDB-FIG-090826-9

Publication

Santoro et al., 2009 - Characterization of vascular mural cells during zebrafish development

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Fig. 7

Vascular mural cells contribute to the ventral aorta and the bulbus arteriosus. Ninety-six hpf wild-type larvae analyzed for transgelin (a) and acta2 (b) expression. transgelin and acta2 are expressed in the bulbus arteriosus (BA) and ventral aorta (VA). (c) Confocal image of a sagittal section of a 96 hpf Tg(flk1:EGFP)^s843 wild-type larva stained for Transgelin (red) show that vascular MCs surround the VA and contribute to the BA. (d) Confocal image of a transverse section of the VA shows single MCs around the ECs (white dots). (e) Confocal projection of the heart region of a 120 hpf Tg(flk1:EGFP)^s843 wild-type larva stained for Transgelin (red). At this stage of development, vascular MCs are present in the BA as well as VA. Scale bar, 20 μm. V, ventricle; L, vascular lumen.

Expression Data

Genes:	acta2 EGFP tagln
Antibody:	Ab1-tagln
Fish:	WT s843Tg
Anatomical Terms:	blood vessel endothelial cell bulbus arteriosus vascular associated smooth muscle cell ventral aorta
Stage Range:	Day 4 to Day 5

Expression Detail

Gene	Fish	Conditions	Stage	Anatomy	Assay
acta2	WT	standard conditions	Day 4	bulbus arteriosus	ISH
acta2	WT	standard conditions	Day 4	ventral aorta	ISH
EGFP	s843Tg	standard conditions	Day 4	blood vessel endothelial cell	IFL
EGFP	s843Tg	standard conditions	Day 5	blood vessel endothelial cell	IFL
tagln	WT	standard conditions	Day 4	bulbus arteriosus	ISH
tagln	WT	standard conditions	Day 4	ventral aorta	ISH

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Antibody Labeling

Antibody	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
Ab1-tagln	s843Tg	standard conditions	Day 4		vascular associated smooth muscle cell	IHC
Ab1-tagln	s843Tg	standard conditions	Day 5		vascular associated smooth muscle cell	IHC

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Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 126(8-9), Santoro, M.M., Pesce, G., and Stainier, D.Y., Characterization of vascular mural cells during zebrafish development, 638-649, Copyright (2009) with permission from Elsevier. Full text @ Mech. Dev.